2014 control sirna software  (Nikon)

Journal: Immunity

Article Title: The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis

doi: 10.1016/j.immuni.2018.08.016

Figure Lengend Snippet: Key Resources Table

Article Snippet: Lab Described in current manuscript Experimental Models: Organisms/Strains C57BL/6 mice Jackson Laboratories 000664 Casp11 −/− mice University of Pittsburgh Animal Housing Described in current manuscript Nlrp3 −/− mice Genentech Inc Mariathasan et al., 2006 Asc −/− mice Genentech Inc Mariathasan et al., 2006 Gsdmd −/− mice University of Pittsburgh Animal Housing He et al., 2015 Ager −/− mice University of Pittsburgh Animal Housing Wendt et al., 2003 Tlr4 −/− mice University of Pittsburgh Animal Housing Deng et al., 2013 B6.129P2-Lyz2tm1(cre)Ifo/J Jackson Laboratories 004781 B6.Cg- Speer6-ps1Tg(Alb-cre)21Mgn/ J Jackson Laboratories 003574 Tlr4 fl/fl mice University of Pittsburgh Animal Housing Deng et al., 2013 Casp11 fl/fl mice University of Pittsburgh Animal Housing Described in current manuscript Hmgb1 fl/fl mice University of Pittsburgh Animal Housing Described in current manuscript Oligonucleotides 5′-ACT TTC TCT CTT CTC ACT −3′, 5′-TGT CTA ACT ATA TTG AAA TGT G-3′ Invitrogen Casp11 -specific primers TCTACACTATAGTCCAGACCC Shi et al., 2014 CASP4 -specific siRNA GTCTGGACTATAGTGTAGATG Shi et al., 2014 CASP4 -specific siRNA CGTACGCGGAATACTTCGA Shi et al., 2014 Control siRNA Software and Algorithms Graphpad Prism 5 software Graphpad Prism 5 software N/A Adobe Illustrator CC 2015 Adobe N/A NIS Elements software Nikon Instruments N/A Microsoft Excel Microsoft N/A Open in a separate window Contact for Reagent and Resource Sharing Further information and requests for reagents may be directed to and will be fulfilled by the Lead Contact, Ben Lu ( nc.ude.usc@ulnebyx ).

Techniques: In Vivo, Enzyme-linked Immunospot, Recombinant, Transfection, In Situ, Enzyme-linked Immunosorbent Assay, Selection, Control, Software